Installing FijiFISH

Author

Kaitlin Sullivan

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Video Tutorial

The FijiFISH macro package is installed as a dropdown menu on your Fiji toolbar. It can either be permanently or temporarily installed.

See below for written instructions.

Permanent

This installation ensures each time you open up FIJI the menu is already installed. To update you will simply replace the existing code with the new version.
1. Copy the raw contents of the latest version of the menu
2. Open your FIJI app
3. Plug-ins -> Macros -> Startup Macros
4. Paste the code at the very bottom of the StartupMacros.txt
5. Download LUT files and put in the FIJI lut folder (only if you want to use the HiPlex Overlay function)
6. Close and restart your FIJI app
7. You will now see the happy hippocampus icon in your FIJI!

Temporary

This installation is useful for using the menu when on shared computers or read-only versions of FIJI. You will need to re-install the macro each time you open a new FIJI session.
1. Save the latest version of the menu as a .ijm file
2. Open your FIJI app
3. From the top menu select: Plug-ins -> Macros -> Install
4. Select FijiFISH-v5.ijm from wherever you have it saved
5. You will now see the happy hippocampus icon in your FIJI for the remainder of your session. 6. HiPlex Overlay currently does not work on read-only versions of FIJI

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